Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse His6Interleukin-25/IL-17E (mHis6IL-25) #11996

No. Size Price
11996LC 50 µg ( With Carrier ) ¥6,860.00 现货查询 购买询价
11996LF 50 µg ( Carrier Free ) ¥6,860.00 现货查询 购买询价
11996SC 10 µg ( With Carrier ) ¥2,321.00 现货查询 购买询价
11996SF 10 µg ( Carrier Free ) ¥2,321.00 现货查询 购买询价
11996 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse His6IL-25 (mHis6IL-25) Val17-Ala169 (Accession #NP_542767) was expressed in human 293 cells at Cell Signaling Technology.

重组小鼠 His6IL-25 (mHis6IL-25) Val17-Ala169 (Accession #NP_542767) 通过Cell Signaling Technology公司的人源293细胞表达。

Molecular Characterization

Recombinant N-terminally His6-tagged mIL-25 has a calculated MW of 20,051 Da. DTT-reduced protein migrates as a 24-31 kDa polypeptide. The nonreduced cystine-linked homodimer migrates as a 46-50 kDa protein. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino terminus of recombinant mHis6IL-25 was verified by amino acid sequencing.

重组的N-端His6标签mIL-25蛋白,其分子量据推算为20,051 Da,DTT-还原处理后的蛋白在24-31kDa之间,未还原的胱氨酸连接的同源二聚体在46-50kDa之间。其在SDS-PAGE胶中的低迁移性和异质性与糖基化有关。重组小鼠His6IL-25蛋白的氨基酸末端通过氨基酸测序得到。

Purity

>97% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant mHis6IL-25. All lots are greater than 97% pure.

6 μg 还原 (+) 和 非还原的(-) 重组小鼠His6IL-25通过SDS-PAGE检测,纯度大于97%,所有批次的纯度均高于97%。

Bioactivity

The bioactivity of recombinant mHis6IL-25 was determined in mouse splenocyte and HT-29 cell cytokine secretion assays. The ED50 of each lot is between 0.5-1.5 ng/ml in mouse splenocyte cells and 0.25-1.5 ng/mL in HT-29 cells.

重组小鼠His6IL-25 的生物活性是通过小鼠脾细胞和HT-29细胞的因子分泌试验确定的。每个样品的ED50在0.5-5 pg/ml之间(小鼠脾细胞)和0.25-1.5 ng/mL之间(HT-29细胞)。

Bioactivity

Bioactivity

IL-13 production from mouse splenocytes cultured with mHis6IL-25 was assessed. Mouse splenoyctes were treated with increasing concentrations of mHis6IL-25 for 72 hr. Cell supernatants were then harvested and assayed for IL-13 by ELISA, and the OD450 was determined. 用mHis6IL-25培养小鼠脾细胞,评估其产生IL-13的效果。小鼠脾细胞通过递增浓度的mHis6IL-25处理72小时。收集细胞上清,并且通过ELISA和OD450分析IL-13。

Coomassie Gel

Coomassie Gel

The purity of recombinant mHis6IL-25 was determined by SDS-PAGE of 6 µg reduced (+) and nonreduced (-) recombinant mHis6IL-25 and staining overnight with Coomassie Blue. 通过6 µg 还原 (+)和非还原的 (-) 的重组mHis6IL-25的SDS-PAGE,检测mHis6IL-25的纯度,考马斯亮蓝过夜染色。

Bioactivity

Bioactivity

GRO α production from HT-29 cells cultured with mHis6IL-25 was assessed. HT-29 cells were treated with increasing concentrations of mHis6IL-25 for 24 hr. Cell supernatants were then harvested and assayed for GRO α by ELISA, and the OD450 was determined. 用mHis6IL-25培养HT-29细胞,评估其产生GRO α效果。HT-29细胞使用递增浓度的mHis6IL-25处理24小时。收集细胞上清,并且通过ELISA和OD450检测分析GRO α。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mHis6IL-25.

每微克His6IL-25内毒素含量少于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of mHis6IL-25 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg mHis6IL-25. Carrier free: Lyophilized from a 0.22 μm filtered solution of mHis6IL-25 in 20 mM Tris, pH 7.2.

有载体:每微克His6IL-25溶解在包含 20 μg BSA的20 mM PBS (pH 7.2)溶液中,并通过 0.22 μm 滤膜过滤后冻干。

无载体:每微克His6IL-25溶解在20 mM PBS (pH 7.2)溶液中,,并通过 0.22 μm 滤膜过滤后冻干。

Background

IL-25, also known as IL-17E, is a member of the IL-17 superfamily of cytokines. IL-25 is expressed in epithelial cells, CD4+ T cells, mast cells, and eosinophils (1). Many cell types are responsive to IL-25, including T cells, macrophages, and epithelial cells (1). The receptor for IL-25 consists of a heterodimer of IL-17RA and IL-17RB (1,2). IL-25 promotes Th2 type immune responses by induction of IL-5, IL-4, and IL-13 and may contribute to allergic inflammation and asthma (1-3). IL-25 has also been shown in research to promote Th9 cell activation and induce apoptosis in breast cancer cells (4,5).

IL-25,又称IL-17E,是IL-17超家族细胞因子成员之一。IL-25在上皮细胞,CD4+T细胞,肥大细胞以及嗜酸性粒细胞中表达(1)。多种细胞类型都可以对IL-25产生应答,如T细胞,巨噬细胞和上皮细胞(1)。IL-25受体是由IL-17RA和IL-17RB组成的异源二聚体(1,2)。IL-25能够通过诱导IL-5、IL-4和IL-13促进Th2型免疫应答,同时可能有助于过敏性炎症和哮喘的发生(1-3)。IL-25同样能够促进Th9细胞的活化以及诱导乳腺癌细胞的凋亡(4,5)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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