Product Pathways - Growth Factors/Cytokines
Mouse His6Interleukin-25/IL-17E (mHis6IL-25) #11996
|11996LC||50 µg ( With Carrier )||￥6,860.00 现货查询||购买询价|
|11996LF||50 µg ( Carrier Free )||￥6,860.00 现货查询||购买询价|
|11996SC||10 µg ( With Carrier )||￥2,321.00 现货查询||购买询价|
|11996SF||10 µg ( Carrier Free )||￥2,321.00 现货查询||购买询价|
|11996||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Source / Purification
Recombinant mouse His6IL-25 (mHis6IL-25) Val17-Ala169 (Accession #NP_542767) was expressed in human 293 cells at Cell Signaling Technology.
重组小鼠 His6IL-25 (mHis6IL-25) Val17-Ala169 (Accession #NP_542767) 通过Cell Signaling Technology公司的人源293细胞表达。
Recombinant N-terminally His6-tagged mIL-25 has a calculated MW of 20,051 Da. DTT-reduced protein migrates as a 24-31 kDa polypeptide. The nonreduced cystine-linked homodimer migrates as a 46-50 kDa protein. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino terminus of recombinant mHis6IL-25 was verified by amino acid sequencing.
>97% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant mHis6IL-25. All lots are greater than 97% pure.
6 μg 还原 (+) 和 非还原的(-) 重组小鼠His6IL-25通过SDS-PAGE检测，纯度大于97%，所有批次的纯度均高于97%。
The bioactivity of recombinant mHis6IL-25 was determined in mouse splenocyte and HT-29 cell cytokine secretion assays. The ED50 of each lot is between 0.5-1.5 ng/ml in mouse splenocyte cells and 0.25-1.5 ng/mL in HT-29 cells.
重组小鼠His6IL-25 的生物活性是通过小鼠脾细胞和HT-29细胞的因子分泌试验确定的。每个样品的ED50在0.5-5 pg/ml之间（小鼠脾细胞）和0.25-1.5 ng/mL之间（HT-29细胞）。
IL-13 production from mouse splenocytes cultured with mHis6IL-25 was assessed. Mouse splenoyctes were treated with increasing concentrations of mHis6IL-25 for 72 hr. Cell supernatants were then harvested and assayed for IL-13 by ELISA, and the OD450 was determined. 用mHis6IL-25培养小鼠脾细胞，评估其产生IL-13的效果。小鼠脾细胞通过递增浓度的mHis6IL-25处理72小时。收集细胞上清，并且通过ELISA和OD450分析IL-13。
The purity of recombinant mHis6IL-25 was determined by SDS-PAGE of 6 µg reduced (+) and nonreduced (-) recombinant mHis6IL-25 and staining overnight with Coomassie Blue. 通过6 µg 还原 (+)和非还原的 (-) 的重组mHis6IL-25的SDS-PAGE，检测mHis6IL-25的纯度，考马斯亮蓝过夜染色。
GRO α production from HT-29 cells cultured with mHis6IL-25 was assessed. HT-29 cells were treated with increasing concentrations of mHis6IL-25 for 24 hr. Cell supernatants were then harvested and assayed for GRO α by ELISA, and the OD450 was determined. 用mHis6IL-25培养HT-29细胞，评估其产生GRO α效果。HT-29细胞使用递增浓度的mHis6IL-25处理24小时。收集细胞上清，并且通过ELISA和OD450检测分析GRO α。
Less than 0.01 ng endotoxin/1 μg mHis6IL-25.
With carrier: Lyophilized from a 0.22 μm filtered solution of mHis6IL-25 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg mHis6IL-25. Carrier free: Lyophilized from a 0.22 μm filtered solution of mHis6IL-25 in 20 mM Tris, pH 7.2.
有载体：每微克His6IL-25溶解在包含 20 μg BSA的20 mM PBS （pH 7.2）溶液中，并通过 0.22 μm 滤膜过滤后冻干。
无载体：每微克His6IL-25溶解在20 mM PBS （pH 7.2）溶液中，，并通过 0.22 μm 滤膜过滤后冻干。
IL-25, also known as IL-17E, is a member of the IL-17 superfamily of cytokines. IL-25 is expressed in epithelial cells, CD4+ T cells, mast cells, and eosinophils (1). Many cell types are responsive to IL-25, including T cells, macrophages, and epithelial cells (1). The receptor for IL-25 consists of a heterodimer of IL-17RA and IL-17RB (1,2). IL-25 promotes Th2 type immune responses by induction of IL-5, IL-4, and IL-13 and may contribute to allergic inflammation and asthma (1-3). IL-25 has also been shown in research to promote Th9 cell activation and induce apoptosis in breast cancer cells (4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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