Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb #11992


No. Size Price
11992S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
11992 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 75 (rodent), 80 (human) Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Monkey, Chicken, Xenopus, Zebrafish, Bovine, Pig,

Specificity / Sensitivity

Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb recognizes endogenous levels of MARCKS protein when phosphorylated at Ser159 and Ser163 (human residues).

当丝氨酸(159 和163位)(人肽段)磷酸化时,Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb识别内源性的总MARCKS蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser159 and Ser163 of human MARCKS protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, serum starved overnight and either DMSO-treated or treated with TPA #4174 (200 nM, 15 min), using Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb.

Western blot方法检测HeLa, NIH/3T3和 C6细胞提取物,细胞血清饥饿过夜后,DMSO处理或TPA #4174 (200 nM, 15 min)处理,使用的抗体为Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb.



Immunoprecipitation of phospho-MARCKS (Ser159/163) from HeLa cells, either DMSO-treated or treated with TPA #4174 (200 nM, 15 min), using Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb (lanes 3 and 4) or MARCKS (D88D11) XP® Rabbit mAb #5607 (lanes 5 and 6). Lanes 1 and 2 represent 10% of input lysate for each treatment. Western blot analysis was performed using MARCKS (D88D11) XP® Rabbit mAb #5607. Note: MARCKS (D88D11) XP® Rabbit mAb #5607 does not immunoprecipitate phospho-MARCKS (Ser159/163) and was used as a negative control.

免疫沉淀法检测HeLa细胞phospho-MARCKS (Ser159/163),DMSO处理或TPA #4174 (200 nM, 15 min)处理,使用的抗体为Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb (泳道3和4) 或 MARCKS (D88D11) XP® Rabbit mAb #5607 (泳道5和6).泳道1和2分别代表每种处理方式中加入的10%的裂解物。进行western blot检测,使用的抗体为MARCKS (D88D11) XP® Rabbit mAb #5607.注意:MARCKS (D88D11) XP® Rabbit mAb #5607不能免疫沉淀phospho-MARCKS (Ser159/163),可作为阴性对照使用。


Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).

豆寇酰化富含丙氨酸C-激酶底物(MARCKS)是一种重要的PKC底物,在多种细胞类型中表达。 MARCKS与细胞活力,细胞粘附,吞噬,膜运输和有丝分裂有关(1)。PKC响应生长因子和氧化应激磷酸化MARCKS的丝氨酸(159,163,167和170位),调节钙/钙调蛋白结合和MARCKS丝状(F)-肌动蛋白交联反应活性(2-4)。PKC磷酸化作用导致MARCKS从细胞膜到细胞质迁移(5)。

  1. Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
  2. Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
  3. Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
  4. Hartwig, J.H. et al. (1992) Nature 356, 618-22.
  5. Thelen, M. et al. (1991) Nature 351, 320-2.

Application References

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