Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Calumenin (4C6) Mouse mAb #11991

No. Size Price
11991S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11991 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 47 Mouse IgG1
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Calumenin (4C6) Mouse mAb recognizes endogenous levels of total calumenin protein.

Calumenin (4C6) Mouse mAb识别内源性的总网腔钙结合蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human calumenin protein.

该单克隆抗体是由针对人网腔钙结合蛋白羧基端的重组蛋白免疫动物制备。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Calumenin (4C6) Mouse mAb.

Western blot方法检测多种细胞系的抽提物,使用的抗体为Calumenin (4C6) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Calumenin (4C6) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

激光共聚焦荧光法检测HeLa细胞,使用的抗体为Calumenin (4C6) Mouse mAb (绿色). 肌动蛋白微丝用DY-554鬼笔环肽标记(红色)。蓝色伪彩色为DNA荧光染料(产品信息为DRAQ5® #4084)。

Background

Calumenin belongs to the CREC family of proteins that also contains reticulocalbin, ERC-55/TCBP-49/E6BP, Cab45, and crocalbin/CBP-50. These EF-hand proteins localize to the endoplasmic reticulum (ER) and are involved in the secretory pathway in mammalian cells (1). Calumenin exists as two isoforms corresponding to alternativly spliced variants of the CALU gene (2). Calumenin has been shown to localize to the ER via a carboxy terminal HDEF ER retention signal (3), and undergoes secretion after trafficking through the secretory pathway (4). Secreted calumenin plays a role in amyloidosis by interacting with serum amyloid P component (5). Calumenin is also a chaperone for mutants of the cystic fibrosis transmembrane conductance regulator (CFTR) (6). Researchers performing a proteomics analysis of endometrial cancer identified calumenin as a potential target for endometrial carcinoma (7).

网腔钙结合蛋白属于CREC蛋白质家族,包含网钙蛋白、ERC-55/TCBP-49/E6BP、Cab45和crocalbin/CBP-50。这些EF-手形蛋白定位于内质网(ER),在哺乳动物细胞中参与分泌途径(1)。网腔钙结合蛋白以两种亚型存在,对应CALU基因的选择性剪接体(2)。已经显示,网腔钙结合蛋白通过羧基末端的HDEF内质网滞留信号定位在ER上(3),通过分泌途径转运后进行分泌(4)。分泌的网腔钙结合蛋白通过与血清淀粉样蛋白P成分相互作用,在淀粉样变性病中起作用(5)。网腔钙结合蛋白也是囊性纤维化跨膜传导调节蛋白(CFTR)突变体的分子伴侣(6)。研究人员对子宫内膜癌进行蛋白质组学分析,发现网腔钙结合蛋白是子宫内膜癌的潜在目标(7)。

  1. Honoré, B. and Vorum, H. (2000) FEBS Lett 466, 11-8.
  2. Jung, D.H. et al. (2006) Biochem Biophys Res Commun 343, 34-42.
  3. Yabe, D. et al. (1997) J Biol Chem 272, 18232-9.
  4. Vorum, H. et al. (1999) Exp Cell Res 248, 473-81.
  5. Vorum, H. et al. (2000) FEBS Lett 465, 129-34.
  6. Teng, L. et al. (2012) PLoS One 7, e40173.
  7. Voisin, S.N. et al. (2011) PLoS One 6, e16352.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications