Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Calumenin (4C6) Mouse mAb #11991

No. Size Price
11991S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11991 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 47 Mouse IgG1
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Calumenin (4C6) Mouse mAb recognizes endogenous levels of total calumenin protein.

Calumenin (4C6) Mouse mAb识别内源性的总网腔钙结合蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human calumenin protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Calumenin (4C6) Mouse mAb.

Western blot方法检测多种细胞系的抽提物,使用的抗体为Calumenin (4C6) Mouse mAb.



Confocal immunofluorescent analysis of HeLa cells using Calumenin (4C6) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

激光共聚焦荧光法检测HeLa细胞,使用的抗体为Calumenin (4C6) Mouse mAb (绿色). 肌动蛋白微丝用DY-554鬼笔环肽标记(红色)。蓝色伪彩色为DNA荧光染料(产品信息为DRAQ5® #4084)。


Calumenin belongs to the CREC family of proteins that also contains reticulocalbin, ERC-55/TCBP-49/E6BP, Cab45, and crocalbin/CBP-50. These EF-hand proteins localize to the endoplasmic reticulum (ER) and are involved in the secretory pathway in mammalian cells (1). Calumenin exists as two isoforms corresponding to alternativly spliced variants of the CALU gene (2). Calumenin has been shown to localize to the ER via a carboxy terminal HDEF ER retention signal (3), and undergoes secretion after trafficking through the secretory pathway (4). Secreted calumenin plays a role in amyloidosis by interacting with serum amyloid P component (5). Calumenin is also a chaperone for mutants of the cystic fibrosis transmembrane conductance regulator (CFTR) (6). Researchers performing a proteomics analysis of endometrial cancer identified calumenin as a potential target for endometrial carcinoma (7).


  1. Honoré, B. and Vorum, H. (2000) FEBS Lett 466, 11-8.
  2. Jung, D.H. et al. (2006) Biochem Biophys Res Commun 343, 34-42.
  3. Yabe, D. et al. (1997) J Biol Chem 272, 18232-9.
  4. Vorum, H. et al. (1999) Exp Cell Res 248, 473-81.
  5. Vorum, H. et al. (2000) FEBS Lett 465, 129-34.
  6. Teng, L. et al. (2012) PLoS One 7, e40173.
  7. Voisin, S.N. et al. (2011) PLoS One 6, e16352.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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