Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Tensin 2 Antibody #11990

No. Size Price
11990S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11990 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 145-155 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Tensin 2 Antibody recognizes endogenous levels of total Tensin 2 protein.

Tensin 2抗体可以识别内源性总Tensin 2蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg155 of human Tensin 2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

多抗由合成肽段免疫动物产生,该肽段与人Tensin 2蛋白Arg155及其邻近肽段序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Tensin 2 Antibody.使用Tensin2抗体对多种细胞提取物进行western blot分析。


Tensin 2 belongs to the Tensin family of cytoskeletal proteins that includes Tensin 1-3 and Cten, which couple integrins to the actin cytoskeleton (1). Tensin proteins contain SH2 and phosphotyrosine binding (PTB) domains, which enable interaction with diverse signaling molecules and proteins. Tensin family proteins play important roles in signal transduction, cell proliferation, and motility (2-5). 
 Tensin 2 is localized to focal adhesions of various tissues with highest expression in the heart, kidney, and liver (6,7). Tensin 2 inhibits Akt/PKB signaling via a phosphatase tensin-type domain (8). However, Tensin 2 also mediates thrombopoietin/c-Mpl signaling, which promotes Akt signaling (9). Interaction with Tensin 2 is essential for the tumor suppressor function of Deleted in Cancer 1 (DLC1) (10-12).

Tensin 2属于细胞骨架蛋白Tensin家族,包括了Tensin 1-3和Cten,它们协助整合素到细胞骨架肌动蛋白(1)。Tensin蛋白包含有SH2和磷酸酪氨酸结合(PTB)结构域,能够使蛋白与各种喜好分子和蛋白质结合。Tensin家族蛋白在信号转导,细胞增殖和运动方面都发挥了重要作用(2-5)。Tensin2位于多种组织的黏着斑区域,在心脏,肾脏和肝脏中高表达(6,7)。Tensin2通过一个磷酸酯酶tensin-样结构域抑制Akt/PKB信号通路(8)。但是,Tensin2也介导了thrombopoietin/c-Mpl信号通路,该通路促进Akt信号(9)。与Tensin2的相互作用对于多种抑癌作用是必须的(10-12)。

  1. Lo, S.H. et al. (1994) Bioessays 16, 817-23.
  2. Lo, S.H. et al. (1994) J Cell Biol 125, 1067-75.
  3. Chen, H. and Lo, S.H. (2003) Biochem J 370, 1039-45.
  4. Katz, M. et al. (2007) Nat Cell Biol 9, 961-9.
  5. Chen, H. et al. (2002) Proc Natl Acad Sci U S A 99, 733-8.
  6. Clark, K. et al. (2010) J Cell Biochem 109, 808-17.
  7. Hafizi, S. et al. (2002) Biochem Biophys Res Commun 299, 793-800.
  8. Hafizi, S. et al. (2005) FASEB J 19, 971-3.
  9. Jung, A.S. et al. (2011) Cell Cycle 10, 1838-44.
  10. Chan, L.K. et al. (2009) PLoS One 4, e5572.
  11. Chen, L. et al. (2012) J Biol Chem 287, 26104-14.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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