Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-BACH1/BRIP1 (Thr1133) Antibody #11983

No. Size Price
11983S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
11983 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 145 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-BACH1/BRIP1 (Thr1133) Antibody recognizes endogenous levels of BACH1/BRIP1 protein only when phosphorylated at Thr1133. This antibody also cross-reacts with a protein of unknown origin at ~130 kDa.Phospho-BACH1/BRIP1 (Thr1133)抗体可以识别1133位苏氨酸磷酸化后的内源性BACH1/BRIP1蛋白。抗体与某未知源性的蛋白在~130kDa处有交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr1133 of human BACH1/BRIP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.多抗由合成肽段免疫动物产生,该肽段与人BACH1/BRIP1蛋白1133位苏氨酸邻近残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with λ phosphatase and calf intestinal phosphatase (CIP) (+), using Phospho-BACH1/BRIP1 (Thr1133) Antibody (upper) or BACH1/BRIP1 Antibody #4578 (lower).使用Phospho-BACH1/BRIP1 (Thr1133) 抗体(上)或 BACH1/BRIP1 抗体 #4578 (下)未处理(-)或使用λ phosphatase和牛小肠碱性磷酸酶(CIP) (+)处理的Jurkat细胞提取物进行western blot分析。

IP

IP

Immunoprecipitation of phospho-BACH1/BRIP1 (Thr1133) from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-BACH1/BRIP1 (Thr1133) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-BACH1/BRIP1 (Thr1133) Antibody.使用正常Rabbit IgG#2729(lane 2)或Phospho-BACH1/BRIP1 (Thr1133) 抗体(lane 3)对293T细胞提取物的磷酸化BACH1/BRIP1 (Thr1133)进行免疫沉淀实验。Lane1使用10%上样量。Phospho-BACH1/BRIP1 (Thr1133)抗体也用于western blot实验。

Background

BACH1, also known as BRIP1 and FANCJ, is a DNA helicase involved in repair of DNA cross-links and double strand breaks (1-3). Interaction between phosphorylated BACH1 and BRCA1 is required for DNA damage-induced checkpoint signaling (3,4). Originally identified as a breast cancer susceptibility gene (1), the BACH1 gene is mutated in Fanconi anemia (5), a recessive disorder characterized by multiple congenital abnormalities, progressive bone marrow failure, and high cancer risk/predisposition. Research investigators have concluded that BACH1 interactions with BRCA1 and the presence of BACH1 mutations in patients with early onset breast cancer indicate that BACH1 may act as a tumor suppressor (6). 
 Phosphorylation of BACH1 at Thr1133 is thought to be involved in regulation of the replication checkpoint and is required for the interaction of BACH1 with TopBP1 (7). BACH1,也被称为BRIP1 和FANCJ,是一种涉及DNA交联和双链损伤修复的DNA解旋酶(1-3)。磷酸化的的BACH1和BRCA1相互作用对于DNA损伤引发的检验点信号通路是必须的(3,4)。BACH1最初被认为是一种乳腺癌敏感基因(1),范科尼贫血病人体内的BRCA1基因会发生突变(5),范科尼贫血是一种隐性遗传的疾病,其特征包括多发性先天异常,渐进的骨髓造血功能衰竭,和癌症高风险/倾向。研究人员已经得出结论BACH1与BRCA1结合,以及在早发乳腺癌病人体内发现的BACH1突变表明BACH1可能作为一种抑癌因子存在(6)。BACH1的1133位苏氨酸磷酸化被认为涉及复制检验点调控和BACH1与TopBP1的相互作用(7)。

  1. Cantor, S.B. et al. (2001) Cell 105, 149-60.
  2. Litman, R. et al. (2005) Cancer Cell 8, 255-65.
  3. Peng, M. et al. (2006) Oncogene 25, 2245-53.
  4. Shiozaki, E.N. et al. (2004) Mol Cell 14, 405-12.
  5. Kennedy, R.D. and D'Andrea, A.D. (2005) Genes Dev 19, 2925-40.
  6. Cantor, S.B. and Guillemette, S. (2011) Future Oncol 7, 253-61.
  7. Gong, Z. et al. (2010) Mol Cell 37, 438-46.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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