Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

LIN28B (D4H1) Rabbit mAb #11965

No. Size Price
11965S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11965 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 32, 21 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

LIN28B (D4H1) Rabbit mAb recognizes endogenous levels of total LIN28B protein.

LIN28B (D4H1)Rabbit mAb兔单抗可以识别内源性的总LIN28B蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LIN28B protein.

此单克隆抗体由合成肽段免疫动物产生,该肽段与人LIN28B蛋白羧基末端的氨基酸残基序列一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA-2, Hep G2, and Huh7 cells using LIN28B (D4H1) Rabbit mAb.

使用LIN28B (D4H1)Rabbit mAb对NTERA-2, Hep G2, 和Huh7细胞提取物进行western blot分析。

IP

IP

Immunoprecipitation of LIN28B from NCCIT cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or LIN28B (D4H1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using LIN28B (D4H1) Rabbit mAb.

使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(lane2)或LIN28B (D4H1)Rabbit mAb (lane 3)对NCCIT细胞中的LIN28B蛋白进行免疫沉淀实验。Lane1是10%上样量。使用LIN28B (D4H1)Rabbit mAb进行western blot分析。

Background

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

LIN28A 和LIN28B是一类发育过程中保守的RNA结合调控蛋白,它抑制let-7家族的miRNAs的处理加工和成熟过程[1,2]。let-7 miRNAs已经显示抑制ras、myc和Hmga2等癌基因[3]。近来LIN28A 和 LIN28B被显示在人类主要肿瘤和肿瘤细胞系中上调表达。它们的过表达直接与let-7 miRNAs下调相关[4]。LIN28基因被报道原生殖细胞发育和精原细胞恶性肿瘤相关[5]。LIN28B的等位基因变异与人类青春期周期调控有关[6]。过表达的LIN28B协同Oct-4, Sox2, 和Nanog可使人成纤维细胞重编程为多潜能ES样细胞[7]。

  1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
  2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
  3. Cho, W.C. (2007) Mol Cancer 6, 60.
  4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
  5. West, J.A. et al. (2009) Nature 460, 909-13.
  6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
  7. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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