Cell Signaling Technology

Product Pathways - DNA Damage

XPD (D3Z6I) Rabbit mAb #11963

ERCC2   TFIIH  

No. Size Price
11963S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11963 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 80 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

XPD (D3Z6I) Rabbit mAb recognizes endogenous levels of total XPD protein.XPD (D3Z6I)Rabbit mAb可以识别内源性总的XPD蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human XPD protein.单克隆抗体由合成肽段免疫动物产生,该肽段与人XPD蛋白的羧基端邻近残基序列一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using XPD (D3Z6I) Rabbit mAb.使用XPD (D3Z6I)Rabbit mAb对多种细胞提取物进行western blot分析。

IP

IP

Immunoprecipitation of XPD from DLD-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or XPD (D3Z6I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using XPD (D3Z6I) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(第2道)或XPD (D3Z6I) Rabbit mAb(第3道)对DLD-1细胞提取物中的XPD进行免疫沉淀实验。第1道是10%上样量。XPD (D3Z6I) Rabbit mAb也用于western blot实验。

Background

XPB and XPD are ATPase/helicase subunits of the TFIIH complex that are involved in nucleotide excision repair (NER) to remove lesions and photoproducts generated by UV light (1). XPB and XPD are 3’-5’ and 5’-3’ DNA helicases, respectively, that play a role in opening of the DNA damage site to facilitate repair (2,3). XPB and XPD both play an important role in maintaining genomic stability, and researchers have linked mutations of these proteins to Xeroderma Pigmentosum (XP) and Trichothiodystrophy (TTD). XP patients have abnormalities in skin pigmentation and are highly susceptible to skin cancers, while TTD patients exhibit symptoms such as brittle hair, neurological abnormalities, and mild photosensitivity (4). In addition to their role in NER, XPB and XPD are involved in transcription initiation as part of the TFIIH core complex (5). The helicase activity of XPB unwinds DNA around the transcription start site to facilitate RNA polymerase II promoter clearance and initiation of transcription (6). XPD plays a structural role linking core TFIIH components with the cdk-activating kinase (CAK) complex that phosphorylates the C-terminus of the largest subunit of RNA polymerase II, leading to transcription initiation (7).XPB和XPD是TFIIH复合物中ATPase/helicase的亚基,涉及核苷酸剪切修复(NER)以去除UV引起的损伤和光产物(1)。XPB和XPD分别是3’-5’和 5’-3’DNA解旋酶,在打开DNA损伤部位存进修复过程中发挥作用(2,3)。XPB和XPD在维持基因组稳定性过程中都扮演了重要的角色,研究者发现这些蛋白发生突变后会导致着色性干皮病(XP)和硫失养症(TTD)。XP病人皮肤色素异常沉着,易患皮肤癌,TTD病人头发发脆,神经系统异常伴有轻度的光敏性(4)。加上在NER中的作用,XPB和XPD作为TFIIH核心复合物的一部分参与了转录起始(5)。XPB的解旋酶作用能偶在转录起始点解璇DNA促进RNA聚合酶II启动子的清除,引发转录(6)。XPD连接TFIIH核心组分和cdk-激活激酶(CAK)复合物,CAK能够磷酸化RNA聚合酶II的最大亚基的羧基端,XPD的连接作用最终会引发转录起始(7)。

  1. Oksenych, V. and Coin, F. (2010) Cell Cycle 9, 90-6.
  2. Evans, E. et al. (1997) EMBO J 16, 6559-73.
  3. Riedl, T. et al. (2003) EMBO J 22, 5293-303.
  4. Lehmann, A.R. (2003) Biochimie 85, 1101-11.
  5. Drapkin, R. et al. (1994) Nature 368, 769-72.
  6. Holstege, F.C. et al. (1996) EMBO J 15, 1666-77.
  7. Rossignol, M. et al. (1997) EMBO J 16, 1628-37.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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