Cell Signaling Technology

Product Pathways - Development

MAML1 (D3E9) Rabbit mAb #11959

Mastermind   Mastermind-like   Notch  

No. Size Price
11959S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11959 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 130 Rabbit IgG
IP 1:50
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

MAML1 (D3E9) Rabbit mAb recognizes endogenous levels of total MAML1 protein. It does not cross-react with MAML2 or MAML3 protein.

MAML1 (D3E9) Rabbit mAb兔单抗可以识别内源性的总MAML1蛋白。但不与MAML2或MAML3蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAML1 protein.

此单克隆抗体由合成肽段免疫动物产生,该肽段与人MAML1蛋白羧基末端邻近的氨基酸残基序列一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MAML1 (D3E9) Rabbit mAb.

使用MAML1 (D3E9) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。

IP

IP

Immunoprecipitation of MAML1 protein from 293T cell extracts using Rabbit DA1E mAb IgG XP® Isotype Control #3900 (lane 2) or MAML1 (D3E9) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MAML1 (D3E9) Rabbit mAb.

使用Rabbit DA1E mAb IgG XP® Isotype Control #3900 (lane 2) 或 MAML1 (D3E9) Rabbit mAb兔单抗 (lane 3)对293T细胞的MAML1进行免疫沉淀分析。Lane1是10%上样量。使用MAML1 (D3E9) Rabbit mAb进行western blot分析。

Chromatin IP

Chromatin IP

CUTLL1 cells were cultured in media with γ-secretase inhibitor (1 μM) for 3 days and then washed and cultured in fresh media for 3 hr. Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106; cells and 5 µl of MAML1 (D3E9) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChI® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human DTX1 intron 3 primers, SimpleChIP® Human HES4 Promoter Primers #7273, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

CUTLL1经过γ-分泌酶抑制剂 (1 μM)处理3天,冲洗然后用新鲜培养基培养3小时。4 x 106细胞核5µl MAML1 (D3E9) Rabbit mAb或2μl的Normal Rabbit IgG #2729交联然后进行染色质免疫共沉淀实验。富集的DNA使用human DTX1 intron 3 引物,SimpleChIP® Human HES4 Promoter 引物 #7273和SimpleChIP® Human α Satellite Repeat 引物 #4486以real-time PCR的方式定量。各样品沉淀得到的DNA量为相对于input中染色质总量的相对定量,Input中染色质量设定为1。

Background

Mastermind-like (MAML) family of proteins are homologs of Drosophila Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML proteins form complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jκ) to regulate Notch target gene expression (3-5). MAML1 also interacts with myocyte enhancer factor 2C (MEF2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7).

Mastermind-like (MAML)家族蛋白是果蝇Mastermind的同源物。该家族在哺乳动物中有3个成员:MAML1,MAML2,和MAML3 (1,2)。MAML蛋白与Notch(ICN)的细胞内结构域和转录因子CSL (RBP-Jκ)结合形成复合物调控Notch靶基因表达(3-5)。MAML1可以与肌肉细胞增强因子2C(MEF2C)结合调控肌细胞生成(6)。MAML2经常发现在粘表皮样癌病人和乳头状淋巴性腺囊瘤体内,与粘表皮样癌转位基因1(MECT1,也被称为WAMTP1或TORC1)融合(7)。

  1. Wu, L. et al. (2002) Mol. Cell Biol. 22, 7688-7700.
  2. Lin, S.E. et al. (2002) J. Biol. Chem. 277, 50612-50620.
  3. Kitagawa, M. et al. (2001) Mol. Cell Biol. 21, 4337-4346.
  4. Nam, Y. et al. (2003) J. Biol. Chem. 278, 21232-21239.
  5. Nam, Y. et al. (2006) Cell 124, 973-983.
  6. Shen, H. et al. (2006) Genes Dev. 20, 675-688.
  7. Tonon, G. et al. (2003) Nat. Genet. 33, 208-213.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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