Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SMARCC1/BAF155 (D7F8S) Rabbit mAb #11956

sc-9746   SWI/SNF  

No. Size Price
11956S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11956 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 155 Rabbit IgG
IP 1:50
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

SMARCC1/BAF155 (D7F8S) Rabbit mAb recognizes endogenous levels of total SMARCC1/BAF155 protein.

SMARCC1/BAF155 (D7F8S) Rabbit mAb兔单抗能够识别内源的SMARCC1/BAF155的总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly975 of human SMARCC1/BAF155 protein.

该单克隆抗体是采用合成的与人源SMARCC1/BAF155 Gly975残基周围序列相对应的肽段免疫动物而生产的。通过蛋白A和多肽的亲和层析方式纯化获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SMARCC1/BAF155 (D7F8S) Rabbit mAb.Western blot方法检测多种细胞系的提取物,使用的抗体为SMARCC1/BAF155 (D7F8S) Rabbit mAb。

IP

IP

Immunoprecipitation of SMARCC1/BAF155 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SMARCC1/BAF155 (D7F8S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SMARCC1/BAF155 (D7F8S) Rabbit mAb.从 HeLa细胞提取物中免疫沉淀SMARCC1/BAF155,使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或SMARCC1/BAF155 (D7F8S) Rabbit mAb (lane 3)。Lane 1为10% input。 使用SMARCC1/BAF155 (D7F8S)进行Western blot检测。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells, grown in phenol red-free medium and 5% charcoal-stripped FBS for 4 d followed by treatment with β-estradiol (10 nM, 45 min), and either 5 μl of SMARCC1/BAF155 (D7F8S) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用从4 x 106 MCF7细胞中提取的交联过的染色质,以及5 µl SMARCC1/BAF155 (D7F8S) Rabbit mAb 或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀,这些细胞需要在含5%活性炭处理胎牛血清以及无酚红的培养基中培养4天,之后再用β-estradiol 进行处理(10 nM, 45 min)。所用试剂盒为SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。富集到的DNA经过实时PCR定量,所使用引物为SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, 以及SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀得到的DNA量由与input chromatin( 相当于1)的相对信号值来表示。

Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).

ATP依赖的染色质改构复合物在转录、DNA复制和修复等核进程的调控中扮演着重要角色。(1,2)。SWI/SNF染色质改构复合物由超过10个亚单位组成,其中包含一个单分子的BRM 和BRG1作为ATPase的催化亚基。ATPase亚单位激活后会破坏组蛋白-DNA的接触,并改变决定性调控原色对染色质的可接近性。 另外的核心亚基和配体亚基作为骨架以维持其稳定性同时还可作为其与不同转录因子及染色质相互作用的接触界面(2,5)。SWI/SNF与核受体、p53、Rb、BRCA1以及MyoD等转录因子的相互作用能够促进复合体募集到目的基因上以调控基因激活,细胞生长,细胞周期以及分化进程(1,6-9)。

SMARCC1/BAF155 is one of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by BRG1 in vitro (10). SMARCC1 is an essential part of the mouse embryonic stem cell specific SWI/SNF complex (esBAF), which is necessary for early embryogenesis, especially proper brain and visceral endoderm development (11-13).

SMARCC1/BAF155是SWI/SNF复合物的核心亚基之一,该复合物是体外BRG1引起的核小体有效重塑是必需的(10)。SMARCC1是小鼠胚胎干细胞特异性SWI/SNF复合物(esBAF)的一个必需的组成部分,该复合物对于早期胚胎形成尤其是大脑和内脏内胚层的正确发育所必需的(11-13)。

  1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
  2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
  3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
  4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
  5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
  6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
  7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
  8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
  9. Simone, C. (2006) J Cell Physiol 207, 309-14.
  10. Phelan, M.L. et al. (1999) Mol Cell 3, 247-53.
  11. Han, D. et al. (2008) Dev Biol 315, 136-46.
  12. Kim, J.K. et al. (2001) Mol Cell Biol 21, 7787-95.
  13. Schaniel, C. et al. (2009) Stem Cells 27, 2979-91.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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