Cell Signaling Technology

Product Pathways - Neuroscience

Oligophrenin-1 Antibody #11939

No. Size Price
11939S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11939 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 92 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Oligophrenin-1 Antibody recognizes endogenous levels of total Oligophrenin-1 protein.

Oligophrenin-1 Antibody 能够检测内源性的CPT1A总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp647 of human Oligophrenin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是由合成的人源HMOX2/HO-2蛋白天冬氨酸(647位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues and cell lines using Oligophrenin-1 Antibody.

Western blot 检测多种组织和细胞系提取物,使用的抗体是Oligophrenin-1 Antibody兔多抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human Oligophrenin-1 (Oligophrenin-1-Myc; +), using Oligophrenin-1 Antibody.

Western blot方法检测HeLa细胞提取物:空质粒转染(-)或带Myc -标签全长人Oligophrenin-1 (Oligophrenin-1-Myc; +)质粒转染;使用的抗体是Oligophrenin-1 Antibody兔多抗。

Background

Oligophrenin-1 is a RhoGTPase-activating protein encoded by the gene OPHN1 (1). Oligophrenin-1 is composed of an N-terminal BAR domain, a pleckstrin homology domain, a central RhoGAP domain, and three putative C-terminal SH3-binding sites. Oligophrenin-1 plays a role in membrane signaling through interaction of its BAR domain with curved membranes, binding of its pleckstrin homology domain with membrane phosphoinositides, and interaction of the SH3-binding sites with adaptor proteins (1-3). Oligophrenin-1 regulates synaptic vesicle endocytosis (3) and plays an important role in dendritic spine morphogenesis (4). Furthermore, by interacting with the transcription factor Rev-erbα and protecting it from degradation, Oligophrenin-1 participates in the regulation of the circadian rhythm in the hippocampus (5). Research studies have demonstrated an involvement of Oligophrenin-1 in X-linked mental retardation (1).

oligophrenin-1是一个RhoGTP酶激活蛋白,由OPHN1基因编码(1)。oligophrenin-1由一个N-末端BAR结构域,一个PH结构域,一个中央RhoGAP结构域和三个推测的C-末端SH3-结合位点组成。Oligophrenin-1在膜信号通路中起到了重要作用,这是通过其BAR结构域与弧形膜的相互作用, PH结构域与膜磷脂酰肌醇膜的结合以及SH3结合位点与接头蛋白的结合来实现的(1-3)。 oligophrenin-1调节突触小泡内吞作用(3),并在树突棘形态发生中起着重要的作用(4)。此外,通过与转录因子Rev-erbα相互作用并保护其免于降解,Oligophrenin-1参与调节了海马的昼夜节律(5)。研究表明X连锁智力障碍与Oligophrenin-1相关(1)。

  1. Billuart, P. et al. (1998) Nature 392, 923-6.
  2. Khelfaoui, M. et al. (2007) J Neurosci 27, 9439-50.
  3. Nakano-Kobayashi, A. et al. (2009) Curr Biol 19, 1133-9.
  4. Govek, E.E. et al. (2004) Nat Neurosci 7, 364-72.
  5. Valnegri, P. et al. (2011) Nat Neurosci 14, 1293-301.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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