Cell Signaling Technology

Product Pathways - NF-kB Signaling

IKKα (3G12) Mouse mAb #11930


No. Size Price
11930S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11930 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 85 Mouse IgG1
F 1:100
IF-IC 1:3200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

IKKα (3G12) Mouse mAb recognizes endogenous levels of total IKKα protein.

IKKα (3G12) Mouse mAb鼠单抗能够检测内源性IKKα总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to a fragment of human IKKα protein.




Confocal immunofluorescent analysis of HCT 116 (high expression; left) and IGROV-1 (low expression; right) cells using IKKα (3G12) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦分析HCT116细胞(高表达;左图)和IGROV-1细胞(低表达;右图),使用抗体是 IKKα (3G12) Mouse mAb (绿色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IKKα (3G12) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot分析多种细胞系的细胞提取物,使用抗体是 IKKα (3G12) Mouse mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HCT 116 cells using IKKα (3G12) Mouse mAb (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody. 流失细胞术分析HCT116细胞的细胞提取物,使用抗体是 IKKα (3G12) Mouse mAb (蓝色)与 concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (红色)对应。 Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408用来作为二抗。


The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).

NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。大部分因子通过一个经典的信号通路,即磷酸化诱导、蛋白酶体介导降解IκB的方法激活NF-κB (3-7)。此通路中最重要的步骤是激活高分子量的IκB激酶(IKK)复合体,此复合体催化的反应中有三个紧密相关的IKK亚基参与完成,IKKα and IKKβ 作为激酶的催化单元,IKKγ作为调控单元(8,9)。IKK的激活依赖于特定位点的磷酸化:IKKβ活性环中的 Ser177和Ser181位点(IKKα中的ser176和ser180 位点),这些特异性的位点的磷酸化导致蛋白构象的变化从而激活激酶(10-13)。

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Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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