Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb #11927

No. Size Price
11927S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
11927 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 55 Rabbit IgG
IP 1:50
F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Bovine, Dog,

Specificity / Sensitivity

Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb recognizes endogenous levels of IRAK4 protein only when phosphorylated at Thr345 and Ser346. This antibody does not react with single phosphorylated proteins.

Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb 只能检测内源的在Thr345 和 Ser346位点磷酸化的IRAK4 蛋白。此抗体也能与在Ser346位点单磷酸化的IRAK4 蛋白交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr345/Ser346 of human IRAK4 protein.

此多克隆抗体是通过合成人源对应的IRAK4 Thr345/Ser346位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of KARPAS-299 cells, untreated (blue) or treated with Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml, 15 min) (green), using Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.流式细胞术分析KARPAS-299细胞,未处理(蓝色)或Human Interleukin-1β (hIL-1β) #8900处理(绿色),使用抗体是Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414用来作为二抗。

Western Blotting

Western Blotting

Western blot analysis of serum-starved KARPAS-299 cell extracts, untreated (-) or treated with Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml, 15 min; +), using Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (upper) or IRAK4 Antibody #4363 (lower).Western blot分析血清饥饿的KARPAS-299 细胞提取物,未处理(-)或使用Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml, 15 分钟; +),使用抗体是Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb (上图) 或 IRAK4 Antibody #4363 (下图)。

Background

Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).

细胞白介素1(IL-1)受体相关激酶(IRAK)是丝氨酸/酪氨酸特异性激酶,它通过IL-1诱导的方式与IL-1受体发生共沉淀(1)。 哺乳动物的IRAK家族有四个成员(IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4)。IL-1结合到IL-1的I类受体(IL-1RI)而促进形成包含IL-1RI, AcP, MyD88和IRAKs的复合体(2)。IRAK在IL-1刺激后能够在短时间内自磷酸化。接着IRAK从IL-1RI复合体上解离,泛素化并与两个膜结合蛋白 TAB2 和TRAF6结合。IRAK-TRAF6-TAB2复合体释放到细胞质中并激活蛋白激酶的级联反应,包括TAK1、IKKs 和应激活化激酶 (3)。

Upon IL-1R/Toll-Like Receptor ligation, IRAK1 and IRAK4 are rapidly recruited to the receptor by the adaptor MyD88 (4). IRAK1 is phosphorylated by IRAK4 at Thr209 and Thr387 (5), followed by sequential autohyperphosphorylation in various domains.

当配体与IL-1R/Toll样受体(TLR)结合后,IRAK1和IRAK4迅速被MyD88招募(4)。IRAK1在不同结构域发生连续自主磷酸化以后,在Thr209和Thr387处被IRAK4磷酸化(5)。

  1. Dinarello, C.A. (1996) Blood 87, 2095-147.
  2. Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84.
  3. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.
  4. Gottipati, S. et al. (2008) Cell Signal 20, 269-76.
  5. Kollewe, C. et al. (2004) J Biol Chem 279, 5227-36.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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