Cell Signaling Technology

Product Pathways - Protein Stability

RBX1 (D3J5I) Rabbit mAb #11922

No. Size Price
11922S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11922 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 13 Rabbit IgG
IP 1:50
IHC-P 1:2400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

RBX1 (D3J5I) Rabbit mAb recognizes endogenous levels of total RBX1 protein.

RBX1 (D3J5I) Rabbit mAb兔单抗能够检测内源性RBX1总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RBX1 protein.

该单克隆抗体经由合成的围绕人RBX1蛋白近羧基端的氨基酸肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RBX1 (D3J5I) Rabbit mAb.

Western blot方法检测多种细胞系的提取物,使用的抗体为RBX1 (D3J5I) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using RBX1 (D3J5I) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

免疫组化分别检测在对照多肽(左)或抗原特异性多肽(右)存在的情况下石蜡包埋的人结肠癌,所使用的抗体为 RBX1 (D3J5I) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse prostate using RBX1 (D3J5I) Rabbit mAb.

免疫组化检测石蜡包埋的小鼠前列腺组织,所使用的抗体为RBX1 (D3J5I) Rabbit mAb兔单抗。

Background

RING-box protein 1 (RBX1 or ROC1) is an essential component of two distinct but structurally related E3 ubiquitin ligase complexes, the SCF complex and the CBC (VHL) complex (1). RBX1 mediates the neddylation of CUL1, which activates SCF E3 ligase by facilitating the ubiquitin transfer from E2 to substrates (2-4). The RING finger domain of RBX1 is required for ubiquitin ligation (5). Two evolutionarily conserved mammalian RBX family members, RBX1/ROC1 and RBX2/ROC2/SAG, have been identified (5). RBX1 is constitutively expressed and binds to CUL2/VHL, while stress-inducible RBX2 binds to CUL5/SOCS (6).

RING-box protein 1 (RBX1 or ROC1)是两种不同但是结构上相关的E3泛素连接酶复合物SCF复合物以及CBC (VHL)中的一个必需的组分(1)。RBX1能够介导CUL1的neddylation,这可以促进泛素从E2转移到底物从而活化SCF E3连接酶(2-4)。RBX1的RING finger结构域是泛素连接所需要的(5)。两种进化上保守的哺乳动物RBX家族成员,RBX1/ROC1 和 RBX2/ROC2/SAG,已经被鉴定出来(5)。RBX1是组成型表达,能够与CUL2/VHL结合,而RBX2是应激诱导型,能够与CUL5/SOCS结合 (6)。

  1. Zheng, N. et al. (2002) Nature 416, 703-9.
  2. Kamura, T. et al. (1999) Genes Dev 13, 2928-33.
  3. Morimoto, M. et al. (2003) Biochem Biophys Res Commun 301, 392-8.
  4. Pan, Z.Q. et al. (2004) Oncogene 23, 1985-97.
  5. Sun, Y. et al. (2001) Antioxid Redox Signal 3, 635-50.
  6. Gu, Q. et al. (2007) Cancer Res 67, 3616-25.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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