Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Chelerythrine Chloride #11916

apoptosis   Bax   Bcl-xL inhibitor   JNK   oxidative   p38  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization






Chemical structure of chelerythrine chloride.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, serum-starved overnight and untreated or treated with Chelerythrine Chloride (2 hr) at the indicated concentrations, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 (upper) or SAPK/JNK (56G8) Rabbit mAb #9258 (lower).

Western blot方法检测HeLa细胞提取物,细胞经血清饥饿过夜后,不处理(-)或用Chelerythrine Chloride指示浓度处理2h,使用的抗体为Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 (上图) 或SAPK/JNK (56G8) Rabbit mAb #9258 (下图).

Directions for Use

Chelerythrine Chloride is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 1.30 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-20 µM either as a pretreatment for 15 min-1 hr prior to treating with a stimulator or used alone with varying treatment times lasting up to 24 hr.

提供白屈菜赤碱氯化物(Chelerythrine Chloride)冻干粉。将5mg冻干粉溶于1.30ml DMSO,作为10 mM储存液。工作浓度和处理时间长短,取决于期望得到的效果,但通常在用刺激物处理之前,使用1-20μM浓度预处理15分钟至1小时,或单独使用最长长达24小时的不同处理时间。


Chelerythrine is a benzophenanthridine alkaloid that elicits a wide range of biological responses. It was initially reported to be a potent PKC inhibitor (IC50 = 660 nM) through interaction with the catalytic domain, competitive with respect to the phosphate acceptor, noncompetitive with respect to ATP (1), and is often used in research as a means to inhibit PKC activation (2-4). However, subsequent studies have shown that chelerythrine is not a potent PKC inhibitor and its effects are independent of PKC inhibition (5,6). Researchers have demonstrated that chelerythrine activates JNK and p38 through an oxidative stress mechanism (6), inhibits Bcl-xL by preventing Bcl-xL-Bak BH3 peptide binding (IC50 = 1.5 μM) and disrupting the interaction between Bcl-xL and Bax (7), and induces apoptosis (6-8).

白屈菜赤碱(Chelerythrine)是一种苯菲生物碱,可引了广泛的生物学反应。最初是作为一种有效的蛋白激酶C抑制剂(IC 50=660 nm)发现,通过与催化结构域的相互作用,与磷酸受体竞争,不与ATP竞争(1),并通常被作为一种研究抑制PKC活化的手段(2-4)。然而,随后的研究表明,白屈菜赤碱并不是一种强有力的PKC抑制剂,且其效果不依赖于PKC的抑制作用(5,6)。研究人员已经证明,白屈菜赤碱通过氧化应激机制激活JNK和p38(6),通过抑制Bcl-xL-Bak BH3肽段结合(IC50 = 1.5 μM)破坏Bcl-xL 和 Bax的相互作用抑制Bcl-xL (7),诱导凋亡(6-8)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :