Cell Signaling Technology

Product Pathways - NF-kB Signaling

IRF-3 (D6I4C) XP® Rabbit mAb #11904

sc-15991   sc-9082  

No. Size Price
11904S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
11904T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
11904 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 50-55 Rabbit IgG
IP 1:50
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

IRF-3 (D6I4C) XP® Rabbit mAb recognizes endogenous levels of total IRF-3 protein.

IRF-3 (D6I4C) XP® Rabbit mAb兔单抗能够检测内源性IRF-3总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IRF-3 protein.

此单克隆抗体是通过重组人源IRF-3蛋白免疫动物制备的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells, untransfected (left) or transfected with Poly(I:C) (2.5 μg/ml, 6 hr; right), using IRF-3 (D6I4C) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦分析HT-29细胞,未转染(左图)或转染Poly(I:C) (2.5 μg/ml, 6小时; 右图),使用抗体是IRF-3 (D6I4C) XP® Rabbit mAb (绿色)。蓝色= DRAQ5® #4084 (DNA荧光染料)。

IP

IP

Immunoprecipitation of IRF-3 from THP-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IRF-3 (D6I4C) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IRF-3 (D6I4C) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.对THP-1细胞提取物中的IRF-3进行免疫沉淀,使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 IRF-3 (D6I4C) XP® Rabbit mAb (泳道3)。泳道1为10%的加入量。Western blot分析使用的抗体是IRF-3 (D6I4C) XP® Rabbit mAb。Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 和 Anti-mouse IgG, HRP-linked Antibody #7076用来作为二抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IRF-3 (D6I4C) XP® Rabbit mAb. Western blot分析多种细胞系的细胞提取物,使用抗体是IRF-3 (D6I4C) XP® Rabbit mAb。

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).

干扰素调控因子(IRFs)由一个家族的转录调控因子组成,这个家族在 Jak/Stat信号通路中的作用是,在病毒感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自体免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥重要作用。IRF家族包含9个成员:IRF-1、IRF-2、ISGF3γ/p48、IRF-3、IRF-4 (Pip/LSIRF/ICSAT)、IRF-5、IRF-6、IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都具有同源性。IRF家族成员通过与具有相似基序的DNA-结合区域的蛋白相互作用来调控转录,例如IFN刺激的应答元件(ISRE)、IFN consensus序列 (ICS) 和IFN 调控元件(IRF-E) (2)。

IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal Ser and Thr residues (between 385 and 405), leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity (5). During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double-stranded RNA treatment. These events likely play a role in activation of IRF-3 (8).

IRF-3能抑制细胞的生长并在控制先天免疫的基因表达中发挥重要作用(1-4)。在未刺激的细胞中, IRF-3 存在于细胞质中。病毒感染导致IRF-3磷酸化并转位到在细胞核,激活含有IRF-3结合位点的启动子。IRF-3的磷酸化发生在一群位于羧基末端丝氨酸和苏氨酸残基中 (在385 和 405位点之间),这导致了其与p300/CBP共激活蛋白的结合,从而促进DNA的结合和转录的活性(5)。在感染期间, IRF-3很可能是通过包括TLR和IKKε、TBK1激酶复合体在内的一系列的信号通路的活化而被激活(6,7)。在病毒的感染、病毒核蛋白壳表达及双链RNA的处理后,IRF-3在Ser396位点发生磷酸化,这些过程在IRF-3的激活方面是至关重要的(8)。

  1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
  2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
  3. Hiscott, J. et al. (1999) J Interferon Cytokine Res 19, 1-13.
  4. Kim, T.Y. et al. (2003) J Biol Chem 278, 15272-8.
  5. Yoneyama, M. et al. (2002) J Interferon Cytokine Res 22, 73-6.
  6. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
  7. Kopp, E. and Medzhitov, R. (2003) Curr Opin Immunol 15, 396-401.
  8. Servant, M.J. et al. (2003) J Biol Chem 278, 9441-7.

Application References

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