Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb #11899

ndrg   ndrg1  

No. Size Price
11899S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
11899 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 46, 48 Rabbit
IP 1:50
IHC-P 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb recognizes endogenous levels of NDRG1 only when phosphorylated at Ser330.

Phospho-NDRG1 (Ser330) (D3A12)Rabbit mAb兔单抗可以识别Ser330被磷酸化的内源性NDRG1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser330 of human NDRG1 protein.

此单克隆抗体由合成肽段免疫动物产生,该肽段与人NDRG1蛋白Ser330邻近氨基酸残基序列一致。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 [paraffin-embedded LNCaP cell pellets untreated (left) or treated with LY294002 #9901 (right)] using Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb.

使用Phospho-NDRG1 (Ser330) (D3A12)Rabbit mAb对SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101[石蜡包埋LNCaP 细胞涂片, 未处理 (左) 或 LY294002 #9901 处理(右)]进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb.

使用Phospho-NDRG1 (Ser330) (D3A12)Rabbit mAb兔单抗对石蜡包埋的的人结肠癌组织进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb.

使用Phospho-NDRG1 (Ser330) (D3A12)Rabbit mAb对石蜡包埋的人肺癌组织进行免疫组化分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved A549, LNCaP, and MCF7 cells, untreated (-) or insulin and λ phosphatase-treated as indicated (+), using Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb (upper), NDRG1 (D6C2) Rabbit mAb #9408 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

A549, LNCaP, 和 MCF7等细胞经血清饥饿后,如图,经无处理(-)或insulin 和λ 磷酸酶处理后(+),使用Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb (上), NDRG1 (D6C2) Rabbit mAb #9408 (中), or β-Actin (D6A8) Rabbit mAb #8457 (下)对细胞提取物进行Western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from LNCaP cells, serum-starved and insulin-treated, in the absence (-) or presence (+) of NDRG1 derived phospho and nonphosphopeptides using Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb.

LNCaP细胞进行血清饥饿和胰岛素处理,在NDRG1 来源的磷酸化或非磷酸化多肽不存在(-)或存在(+)情况下,使用Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb对细胞提取物进行western blot分析。

Background

N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3.

N-myc下游调控gene1(NDRG1),也被称为Cap43,Drg1,RTP/rit42和Proxy-1,是NDRG家族的一员,该家族包含的4个成员(NDRG1-4)在生长,分化和细胞存活过程中发挥作用(1-5)。广泛表达的NDRG1并对多种压力信号发生应激反应,包括DNA损伤(4),缺氧(5),和镍及钙浓度升高(2)。NDRG1在N-myc缺陷小鼠中表达升高,能够被N-和c-myc负调控(1-6)。在DNA损伤过程中,NDRG1通过p53信号激活,并且是p53介导的凋亡过程中所必须的(4,7)。研究显示,NDRG1可能通过促进分化,抑制生长,调控转移和血管生成等肿瘤进程过程中发挥功能(3,4,6,8,9)。NDRG1基因发生无义突变后会引发遗传性运动和感觉神经病病变-Lom(HMSNL),该结论由证明NDRG1在维持髓鞘和轴突存活的研究支持(10,11)。NDRG1在肥大细胞成熟过程中表达上调,它的缺失会导致衰减的过敏反应(12)。NDRG1和NDRG2都是SGK1的底物,尽管SGK1介导的磷酸化的生理性作用尚未可知(13)。NDRG1可以被SGK1在Thr328, Ser330, Thr346, Thr356,以及Thr366磷酸化。NDRG1被SGK1磷酸化后可以被GSK-3磷酸化。

  1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
  2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9.
  3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92.
  4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
  5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
  6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.
  7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
  8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.
  9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43.
  10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
  11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
  12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.
  13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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