Cell Signaling Technology

Product Pathways - Adhesion

PINCH (5G7) Mouse mAb #11890

No. Size Price
11890S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11890 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 35 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

PINCH (5G7) Mouse mAb recognizes endogenous levels of total PINCH protein. The antibody recognizes a protein of unknown origin at 80 kDa in some cell lines.

PINCH (5G7) Mouse mAb识别内源性的总PINCH蛋白。该抗体识别一些细胞系中未知的80 kDad蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the the human PINCH protein.

该单克隆抗体由人PINCH蛋白特异性的重组蛋白免疫动物产生。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PINCH (5G7) Mouse mAb.Western blot检测多种细胞提取物,使用的抗体是 PINCH (5G7) Mouse mAb。

Background

The extracellular matrix (ECM) is a complex structure of secreted macromolecules surrounding mammalian organs and tissues. Controlled interactions between cells and the ECM are important in proliferation, migration, survival, polarity, and differentiation. Cells contact the ECM primarily through focal adhesion complexes, which contain integrins, as well as multiple adaptor and signaling proteins (1). The ILK/PINCH/Parvin (IPP) adaptor complex acts at the interface of the integrin/actin connection to regulate formation of focal adhesions and integrin signaling. Roles for IPP proteins outside of the IPP complex have been proposed, including regulation of gene expression (2,3). 
 PINCH, also known as LIMS1, has been shown to function as a specific regulator of gene expression in glomerular podocytes in response to TGF-β1 (4). Researchers have shown that PINCH is highly expressed in some human tumors, and that PINCH can promote resistance to ionizing radiation through activation of Akt (5,6).

细胞外基质(ECM)是存在于哺乳动物器官和组织周围的一种复杂的大分子结构。细胞和细胞外基质之间的相互作用对于细胞增殖、移动、存活、极性和分化均有非常重要的作用。细胞最初通过膜内在的异二聚体整合素传递信息。整合素将细胞外基质与细胞骨架连接,进而通过局部的粘附分子蛋白复合物传递细胞信号机制(1)。ILK/PINCH/Parvin (IPP)连接复合物在整合素/肌动蛋白的之间调节局部粘附分子的形成及整合素的信号转导中起作用。IPP复合物之外的IPP蛋白作用已经被提及,包括调节基因表达(2,3)。PINCH,也被认为是LIMS1,被认为是一种特殊的基因表达调节蛋白,在肾小球足细胞中对TGF-β1应答(4)。研究者表明PINCH在一些人肿瘤中高度表达,PINCH能够通过激活Akt提高对电离辐射的抵抗力(5,6)。

  1. Burridge, K. et al. (1988) Annu Rev Cell Biol 4, 487-525.
  2. Legate, K.R. et al. (2006) Nat Rev Mol Cell Biol 7, 20-31.
  3. Wu, C. (2004) Biochim Biophys Acta 1692, 55-62.
  4. Wang, D. et al. (2011) PLoS One 6, e17048.
  5. Eke, I. et al. (2010) J Clin Invest 120, 2516-27.
  6. Sandfort, V. et al. (2010) PLoS One 5, .

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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