Cell Signaling Technology

Product Pathways - Protein Stability

UBE2S (D5H9H) Rabbit mAb #11878

E2   UBE2   Ubiquitin Conjugating Enzymes  

No. Size Price
11878S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11878 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 26 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog,

Specificity / Sensitivity

UBE2S (D5H9H) Rabbit mAb recognizes endogenous levels of total UBE2S protein.

UBE2S (D5H9H) Rabbit mAb兔单抗识别内源性的UBE2S总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBE2S protein.

该单克隆抗体经合成与人氨基末端邻近的氨基酸残基序列一致的肽段,免疫动物产生。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UBE2S (D5H9H) Rabbit mAb.Western blot检测多种细胞提取物。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human UBE2S (hUBE2S-Myc/DDK; +), using UBE2S (D5H9H) Rabbit mAb.Western blot检测mock transfected (-)或转染表达Myc/DDK-tagged人全长UBE2S (hUBE2S-Myc/DDK; +)的293T细胞,采用UBE2S (D5H9H) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), or SignalSilence® UBE2S siRNA Ι #7220 (+), using UBE2S (D5H9H) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The UBE2S (D5H9H) Rabbit mAb confirms silencing of UBE2S expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.Western blot检测转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® UBE2S siRNA Ι #7220 (+)的293T细胞,采用UBE2S (D5H9H) Rabbit mAb (upper)或GAPDH (D16H11) XP® Rabbit mAb #5174 (lower)。UBE2S (D5H9H) Rabbit mAb证实了UBE2S的沉默表达,采用GAPDH (D16H11) XP® Rabbit mAb作为内参照。

IP

IP

Immunoprecipitation of UBE2S from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or UBE2S (D5H9H) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UBE2S (D5H9H) Rabbit mAb.Immunoprecipitation检测293T细胞提取物中的UBE2S,采用Rabbit (DA1E) mAb IgG XP®同型对照#3900 (lane 2) 或UBE2S (D5H9H) Rabbit mAb (lane 3)。Lane 1中样品浓度为10%。Western blot检测采用UBE2S (D5H9H) Rabbit mAb。

Background

Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).

蛋白泛素化作用需要E1, E2, 和E3泛素-结合酶的协同作用。泛素最先通过具有硫酯的泛素激活酶E1通过ATP-依赖形式活化。活化的泛素然后通过含有巯基的泛素载体蛋白E2转运。最后从E2的泛素转运到含有ε-氨基的赖氨酸残基靶蛋白,这由泛素连接酶E3介导(1)。

The human anaphase promoting complex (APC/C) is a large macromolecular E3 ligase complex that is largely responsible for timely progression through mitosis via the sequential targeting of cell cycle regulators for proteasomal degradation. Recent work has revealed that APC/C substrates are marked for proteasomal degradation during cell cycle progression through the covalent assembly of Lys11-linked ubiquitin chains, which occurs through a priming phase and an elongation phase (2-5). The APC/C utilizes, in part, the UBE2C/UBCH10 E2 enzyme to prime substrates for degradation through the covalent attachment of short Lys11-linked chains (3,6). The Lys11-specific elongating E2 enzyme, UBE2S/E2-EPF, extends these short chains into long Lys11-linked ubiquitin chains on APC/C bound substrates (2,3,7). In addition to the well-established biochemical role for UBE2S in cell cycle regulation, researchers have found evidence that this enzyme is overexpressed in many types of human cancer (8), and has been implicated in hypoxia signaling (9,10). Indeed, UBE2S has been reported by researchers to associate with VHL and to target it for proteasomal degradation, thereby stabilizing HIF-1α (9).

人分裂后期促进复合物(APC/C)是一种大分子的E3连接酶复合物,经过对蛋白酶体降解的细胞周期的调节子的连续的靶向作用,对有丝分裂的时序性进程起重要作用。最近的研究表明APC/C的底物通过共价组装Lys11-连接的泛素链,在细胞周期进展中起蛋白酶体的降解作用,主要发生在起始阶段和延伸阶段(2-5)。APC/C部分利用UBE2C/UBCH10 E2酶来介导底物的降解,通过与短的Lys11-linked链共价连接(3,6)。Lys11-特异的延伸的E2酶,UBE2S/E2-EPF, 扩展这些短链到APC/C 结合底物上的Lys11-linked泛素链(2,3,7)。除外在细胞周期调节中的UBE2S的生化作用被明确外,这些酶在许多人癌症中过度表达(8),并在缺氧信号传导中发挥作用(9,10)。确实,UBE2S已经被报道与VHL有关,并是蛋白酶体降解的靶目标,从而稳定了HIF-1α (9)。

  1. Hershko, A. (1988) J Biol Chem 263, 15237-40.
  2. Williamson, A. et al. (2009) Proc Natl Acad Sci U S A 106, 18213-8.
  3. Jin, L. et al. (2008) Cell 133, 653-65.
  4. Wu, T. et al. (2010) Proc Natl Acad Sci U S A 107, 1355-60.
  5. Song, L. and Rape, M. (2010) Mol Cell 38, 369-82.
  6. Summers, M.K. et al. (2008) Mol Cell 31, 544-56.
  7. Wickliffe, K.E. et al. (2011) Cell 144, 769-81.
  8. Tedesco, D. et al. (2007) Neoplasia 9, 601-13.
  9. Jung, C.R. et al. (2006) Nat Med 12, 809-16.
  10. Roos, F.C. et al. (2011) Am J Pathol 178, 853-60.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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