Cell Signaling Technology

Product Pathways - Lineage Markers

LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #11845

No. Size Price
11845S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
11845 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse, Endogenous Rabbit IgG
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey,

Specificity / Sensitivity

LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total LIN28A protein.

LIN28A (D1A1A) XP® Rabbit mAb(Alexa Fluor® 647偶联)可以识别内源性的总LIN28A蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro201 of human LIN28A protein.

此单克隆抗体由合成肽段免疫动物产生,该肽段与人LIN28A蛋白的Pro201邻近氨基酸残基序列一致。

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated LIN28A (D1A1A) XP® Rabbit mAb #8641.

此CST公司抗体偶联Alexa Fluor® 647荧光素染料,并经人类细胞直接流式细胞仪免疫荧光分析进行内部测试。抗体预期和未偶联Alexa Fluor® 647的LIN28A (D1A1A) XP® Rabbit mAb兔单抗#8641显示同样的物种交叉反应。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa (blue) and NTERA-2 cl.D1 (green) cells using LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).

使用LIN28A (D1A1A) XP® Rabbit mAb(Alexa Fluor® 647偶联)对HeLa(蓝色)和NTERA-2 cl.D1(绿色)细胞进行流式分析。

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cl.D1 (left) and HeLa (right) cells using LIN28A (D1A1A) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 Phalloidin #8878 (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

使用LIN28A (D1A1A) XP® Rabbit mAb(Alexa Fluor® 647偶联)(蓝色伪彩)对NTERA-2 cl.D1 (左)和 HeLa (右)细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用Alexa Fluor® 488 Phalloidin 标记#8878(绿色)。红色= 碘化丙啶(PI)/RNase Staining Solution #4087.

Background

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

LIN28A 和LIN28B是一类在发育过程中保守的调控RNA结合蛋白,它抑制let-7家族的miRNAs的处理加工和成熟过程[1,2]。let-7 miRNAs已经显示抑制ras、myc和Hmga2等癌基因[3]。近来研究显示LIN28A 和LIN28B在人类主要肿瘤和肿瘤细胞系中上调表达。它们的过表达直接与下调的let-7 miRNAs正相关[4]。LIN28基因被报道与原生殖细胞发育和精原细胞恶性肿瘤相关[5]。LIN28B的等位基因变异与人类青春期周期调控有关[6]。过表达的LIN28A联合Oct-4, Sox2, 和Nanog可重调人成纤维细胞重编程成多潜能ES样细胞[7]。

  1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
  2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
  3. Cho, W.C. (2007) Mol Cancer 6, 60.
  4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
  5. West, J.A. et al. (2009) Nature 460, 909-13.
  6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
  7. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications