Cell Signaling Technology

Product Pathways - Development

WBP2 Antibody #11831

WBP-2   WW domain binding protein  

No. Size Price
11831S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11831 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 32 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

WBP2 Antibody recognizes endogenous levels of total WBP2 protein.

WBP2抗体可以识别内源性的总WBP2蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn239 of human WBP2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

多抗由合成肽段免疫动物产生,该肽段与人WBP2蛋白Asn239邻近氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using WBP2 Antibody.

使用WBP2抗体对多种细胞提取物进行western blot分析。

Background

WW-domain binding protein 2 (WBP2) is an adaptor protein first identified in a screen for proteins that interact with YAP1 (1). WBP2 was subsequently shown to bind other WW domain-containing proteins, including TAZ and NEDD4-like ubiquitin-protein ligases (2-5). There is strong evidence for a conserved functional role for WBP2 in the Hippo kinase tumor suppressor pathway. WBP2 interaction is required for the oncogenic properties of TAZ in breast cancer cells (4) and the growth-promoting properties of the YAP homolog Yorkie in Drosophila (6). In vitro studies have also suggested that WBP2 may act as a transcriptional co-activator of both estrogen and progesterone receptors (7); this interaction was shown to be dependent upon tyrosine phosphorylation of WBP2 by c-Src and c-Yes kinases (8).

WW-结构域结合蛋白2(WBP2)是一种接头蛋白,最初在对YAP1结合蛋白进行筛选时发现(1)。WBP2随后发现能够与其他的含有WW-结构域蛋白结合,包括TAZ和NEDD4样泛素蛋白连接酶(2-5)。有强有力的证据表明WBP2在Hippo激酶抑癌通路中发挥了功能。与WBP2的结合对于TAZ在乳腺癌细胞致癌作用(4)及果蝇中YAP同源物Yorkie的生长促进作用都是必须的(6)。体外实验提示WBP2可能是雌激素和孕激素的转录共激活因子(7);这种结合依赖于c-Src和c-Yes激酶对于WBP2的酪氨酸的磷酸化(8)。

  1. Chen, H.I. and Sudol, M. (1995) Proc Natl Acad Sci U S A 92, 7819-23.
  2. Chen, H.I. et al. (1997) J Biol Chem 272, 17070-7.
  3. McDonald, C.B. et al. (2011) Biochemistry 50, 9616-27.
  4. Chan, S.W. et al. (2011) Oncogene 30, 600-10.
  5. Jolliffe, C.N. et al. (2000) Biochem J 351 Pt 3, 557-65.
  6. Zhang, X. et al. (2011) Cell Death Differ 18, 1346-55.
  7. Dhananjayan, S.C. et al. (2006) Mol Endocrinol 20, 2343-54.
  8. Lim, S.K. et al. (2011) FASEB J 25, 3004-18.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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