Cell Signaling Technology

Product Pathways - Metabolism

Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb #11818

ACAC   ACACA   ACACB   ACC   ACC-alpha   ACC1   ACC2   ACCA   ACCB   Acetyl-CoA Carboxylase   Acetyl-CoA carboxylase 1   Acetyl-CoA carboxylase 2  

No. Size Price
11818S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
11818T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
11818 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 280 Rabbit IgG
IP 1:50
IHC-P 1:400
IF-IC 1:125

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79.

Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗能够检测内源性的在79位丝氨酸磷酸化的乙酰辅酶A羧化酶蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein.

该单克隆抗体是由合成的人源酰辅酶A羧化酶蛋白丝氨酸(79位)的肽段免疫动物而制备的。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NCI-H2228 cell pellets, untreated (left) or phenformin-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

免疫组织化学分析石蜡包埋的NCI-H2228细胞,未经处理(左)或苯乙双胍处理(右),使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb.

免疫组织化学分析石蜡包埋的人肺癌细胞,未经处理(左)或λ磷酸酶处理(右),使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with Oligomycin #9996 (0.5 μM, 30 min), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment.

Western blot方法检测SH-SY5Y细胞提取物:未处理或使用Oligomycin #9996 处理(0.5 μM, 30 min),使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗 (上图)或 Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗 #3676(下图)。使用λ 磷酸酶验证抗体的磷酸化特异性。

IF-IC

IF-IC

Confocal immunofluorescent analysis of 293 cells (all nutrient-starved with Krebs-Ringer bicarbonate buffer for 4 hr), starved only (top left), serum-treated (10%, 30 min; top right), H2O2-treated (10 mM, 10 min; bottom left), or λ phosphatase-treated (2 hr; bottom right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析293细胞(所有饥饿处理为Krebs-Ringer碳酸氢盐缓冲液处理4hr),仅饥饿(左上),血清处理(10%, 30min;右上),过氧化氢处理(10 mM, 10min;左下),或λ磷酸酶处理(2hr,右下),用Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗(绿色)(Ser79)。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. 免疫组织化学分析石蜡包埋的人乳腺癌细胞,使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. 免疫组织化学分析石蜡包埋的人肺癌细胞,未经处理(左)或λ磷酸酶处理(右),使用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb。

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5)。

乙酰辅酶A羧化酶(ACC)催化乙酰辅酶A羧化形成丙二酰辅酶A(1)。这是脂肪酸生物合成和氧化过程中的关键酶(1)。在啮齿类动物中,脂肪组织中主要表达的是265 kDa的ACC1(ACCα),而280 kDa的ACC2(ACCβ)是氧化组织中的主要构型(1,2)。然而,在人体中,ACC2在脂肪与氧化组织均是主要构型(1,2)。AMPK催化的Ser79磷酸化与PKA催化的 Ser1200磷酸化会抑制ACC的酶活性(3)。ACC是抗肥胖药物的潜在靶点(4,5)。

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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