Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb #11817

Grb-10   GRB-IR   GRB10   GRBIR   Growth factor receptor-bound protein 10   Insulin receptor-binding protein Grb-IR   IRBP   KIAA0207   MEG1   RSS  

No. Size Price
11817S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
11817 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 80 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb recognizes endogenous levels of GRB10 protein only when phosphorylated at Ser476.

Phospho-GRB10 (Ser476) (D4E6)mAb兔单抗可以识别Ser476被磷酸化的内源性GRB10蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser476 of human GRB10 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved NIH/3T3 cells, untreated or treated with insulin (100 nM, 5 min), hIGF-1 #8917 (100 ng/ml, 5 min), or λ phosphatase, using Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

血清饥饿后的NIH/3T3不处理或经过胰岛素(100 nM, 5 min), hIGF-1 #8917 (100 ng/ml, 5 min), 或 λ phosphatase处理后获得细胞提取物,使用Phospho-GRB10 (Ser476) (D4E6)Rabbit mAb兔单抗(上)或β-Actin (D6A8) Rabbit mAb 兔单抗#8457(下)进行western blot分析。



Immunoprecipitation of phospho-GRB10 (Ser476) from extracts of serum starved NIH/3T3 cells treated with insulin (100 nM, 5 min), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb.

血清饥饿后的NIH/3T3细胞经过胰岛素(100 nM, 5 min)处理获得提取物,使用Rabbit mAb IgG XP® Isotype Control #3900 (lane 2)或Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb 兔单抗(lane 3)对phospho-GRB10 (Ser476)进行免疫沉淀。Lane1是10%上样量。使用phospho-GRB10 (Ser476) (D4E6)mAb兔单抗进行western blot分析。


Recently GRB10 was shown to be a direct substrate of mTORC1 (5). It is phosphorylated by mTORC1 at Ser150, Ser428, and Ser476 upon insulin stimulation (5).The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS, and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules including Raf, Akt, and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4).

近期,GRB10被认为是mTORC1的直接底物(5)。胰岛素刺激后,mTORC1可以磷酸化GRB10的Ser150, Ser428, 和Ser476(5). GRB7接头蛋白家族由GRB7, GRB10和GRB14组成,都含有一个氨基末端富含脯氨酸SH3结合结构域,后面紧随着PH, PBS, 和 SH2结构域。每个家族成员都有多个剪切异构体(1)。有报道表明GRB10可以和多种受体酪氨酸激酶(RTKs)以及下游信号分子包括Raf,Akt和Nedd4结合(1,2)。尽管最初认为GRB10只能在丝氨酸残基处磷酸化(3),Src激酶家族成员可以磷酸化GRB10的Tyr67(4)。

Application References

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