Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

DEPTOR/DEPDC6 (D9F5) Rabbit mAb #11816

DEP domain containing 6   DEP domain containing mTOR interacting protein   DEP domain-containing mTOR-interacting protein   DEP domain-containing protein 6   DEP.6   DEPD6   DEPDC6   Deptor   DKFZp564B1778   FLJ12341   FLJ12428   FLJ13854  

No. Size Price
11816S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11816 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat,Monkey, Endogenous 48 Rabbit IgG
IP 1:50
IHC-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

DEPTOR/DEPDC6 (D9F5) Rabbit mAb recognizes endogenous levels of total DEPTOR/DEPDC6 protein.

DEPTOR/DEPDC6 (D9F5)Rabbit mAb兔单抗可以识别内源性总的DEPTOR/DEPDC6 (D9F5)蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu113 of human DEPTOR/DEPDC6 protein.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human thyroid using DEPTOR/DEPDC6 (D9F5) Rabbit mAb.

使用DEPTOR/DEPDC6 (D9F5) Rabbit mAb兔单抗对石蜡包埋的人甲状腺组织进行免疫组化分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from RPMI 8226, MCF7, and INS-1 cells using DEPTOR/DEPDC6 (D9F5) Rabbit mAb.

使用DEPTOR/DEPDC6 (D9F5) Rabbit mA兔单抗对RPMI 8226, MCF7和 INS-1细胞提取物进行western blot分析。


DEPTOR/DEPDC6 is a component of both mTORC1 and mTORC2 complexes (1). It interacts with mTOR and inhibits mTORC1 and mTORC2 kinase activities (1). mTOR, along with casein kinase I, phosphorylates DEPTOR/DEPDC6 in the presence of growth signals, which leads to the degradation of DEPTOR/DEPDC6 (2). Research studies have shown that transgenic mice overexpressing DEPTOR/DEPDC6 have more white adipose tissue, and DEPTOR/DEPDC6 expression levels increase in the white adipose tissue of obese humans (3). Furthermore, the expression of DEPTOR/DEPDC6 is induced during mouse adipocyte differentiation (3). Together these findings suggest that DEPTOR/DEPDC6 regulates adipogenesis (3).


  1. Peterson, T.R. et al. (2009) Cell 137, 873-86.
  2. Gao, D. et al. (2011) Mol Cell 44, 290-303.
  3. Laplante, M. et al. (2012) Cell Metab 16, 202-12.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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