Cell Signaling Technology

Product Pathways - Metabolism

ATF-4 (D4B8) Rabbit mAb #11815

Activating transcription factor 4   activating transcription factor 4 (tax-responsive enhancer element B67)   ATF-4   ATF4   cAMP response element-binding protein 2   cAMP-dependent transcription factor ATF-4   cAMP-responsive element-binding protein 2   CREB-2   CREB2   Cyclic AMP-dependent transcription factor ATF-4   Cyclic AMP-responsive element-binding protein 2   DNA-binding protein TAXREB67   sc-200   Tax-responsive enhancer element-binding protein 67   TaxREB67   TXREB  

No. Size Price
11815S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
11815 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 49 Rabbit IgG
IP 1:50
IF-IC 1:200
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

ATF-4 (D4B8) Rabbit mAb recognizes endogenous levels of total ATF-4 protein.

ATF-4 (D4B8) Rabbit mAb能够检测内源性ATF-4总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATF-4 protein.

该单克隆抗体是由合成的人源 ATF-4蛋白C末端肽段免疫动物而制备的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or tunicamycin-treated (2 μg/ml, 8 hr; right), using ATF-4 (D4B8) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

共聚焦免疫荧光分析HeLa 细胞,未处理(左图)或衣霉素处理 (2 μg/ml, 8 hr; 右图),所用抗体为 ATF-4 (D4B8) Rabbit mAb 兔单抗(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and HeLa cells, untreated (-) or tunicamycin-treated (2 μg/ml, 8 hr; +), using ATF-4 (D4B8) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot方法检测293和HeLa细胞提取物:未经处理 (-) 和衣霉素处理(2 μg/ml, 8 hr; +),使用的抗体是ATF-4 (D4B8) Rabbit mAb兔单抗 (上图) 或 β-Actin (D6A8) Rabbit mAb兔单抗 #8457 (下图)。

IP

IP

Immunoprecipitation of ATF-4 from extracts of 293 cells, treated with tunicamycin (2 μg/ml, 8 hr), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ATF-4 (D4B8) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ATF-4 (D4B8) Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.

免疫沉淀方法检测293细胞提取物ATF-4蛋白,使用衣霉素处理(2 μg/ml, 8 hr),使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或ATF-4 (D4B8) Rabbit mAb (泳道3)。泳道1为10%对照。使用ATF-4 (D4B8) Rabbit mAb 进行Western blot方法分析,二抗是Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 和Anti-mouse IgG, HRP-linked Antibody #7076。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MEF wild-type cells treated with tunicamycin (2ug/ml) overnight, and 5 µl of ATF-4 (D4B8) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

ATF-4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway (1-3). ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4 (2). Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress (4).

ATF-4是激活转录因子/ cAMP反应元件结合蛋白家族成员,在PERK和eIF2α内质网应激反应途径中发挥重要作用(1-3)。ER应激抑制了大多数mRNA的翻译,但也选择性地刺激了某些基因如ATF-4的翻译(2)。诱导ATF-4表达会增加基因的表达量,这对于从ER应激状态中恢复正常是非常重要的(4)。

  1. Fawcett, T.W. et al. (1999) Biochem J 339 ( Pt 1), 135-41.
  2. Harding, H.P. et al. (2000) Mol Cell 6, 1099-108.
  3. van Huizen, R. et al. (2003) J Biol Chem 278, 15558-64.
  4. Yusta, B. et al. (2006) Cell Metab 4, 391-406.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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