Cell Signaling Technology

Product Pathways - Phosphatases

PP2A C Subunit Blocking Peptide #1067

prophos  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

This peptide is used to block PP2A C Subunit (52F8) Rabbit mAb #2259 and PP2A C Subunit Antibody #2038 reactivity in immunohistochemistry protocols. 该多肽用于阻断免疫组织化学方法中PP2A C Subunit (52F8) Rabbit mAb #2259和PP2A C Subunit Antibody #2038的反应活性。

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks PP2A C Subunit (52F8) Rabbit mAb #2259 and PP2A C Subunit Antibody #2038 signal in immunohistochemistry.该多肽的质量由反相高效液相色谱和质谱技术监控。该多肽能够在免疫组织化学实验中封闭PP2A C Subunit (52F8) Rabbit mAb #2259和PP2A C Subunit Antibody #2038的信号。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PP2A C Subunit Antibody #2038 in the presence of control peptide (left) or PP2A C Subunit Blocking Peptide (right).免疫组织化学方法检测石蜡包埋人类乳腺癌组织,使用的抗体为PP2A C Subunit Antibody #2038,左图为与对照多肽共孵育,右图为PP2A C Subunit Blocking Peptide 共孵育。

Applications

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.作为阻断剂以衡量免疫组织化学方法中抗体反应的特异性。

Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.对于免疫组织化学,在100μl总体积中,所加多肽和抗体的比为2:1。孵育至少30分钟后将100μl液体全部加至切片。推荐抗体稀释度参见产品信息单页。

Background

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8). 蛋白磷酸酶2A (PP2A)是一种重要的蛋白丝氨酸/苏氨酸磷酸酶,在所有的真核细胞中结构保守。PP2A在各种细胞信号转导通路中都是关键的酶,它调节基本的细胞活动如DNA复制、转录、翻译、代谢、细胞周期进程、细胞分化、细胞凋亡和发育(1-3)。核心的酶由催化性的C亚基和调节性的A亚基(PR65)组成,每个亚基都包括α 和β异构体(1)。另外的亚基属于四个不相关蛋白的不同家族。B (PR55)和B'调节蛋白家族都包含α,β,γ 和δ异构体,而B'还含有ε蛋白。B''家族包括PR72,PR130,PR59 和PR48异构体,而纹蛋白(PR110)和SG2NA (PR93)都属于B'''调节蛋白家族。这些B亚基竞争性的结合核心亚基A上面的一个共同结合位点(1)。该全酶的可变阵列,尤其是调节性B亚基,允许PP2A具有多种功能。PP2A的功能受其表达、分布、全酶构成和转录后修饰的调控。Src介导的PP2A第307位酪氨酸磷酸化响应EGF或者胰岛素,最终导致PP2A的活性显著下降(4)。已观察到PP2A的第309位亮氨酸的羧基官能团可发生可逆的甲基化修饰(5,6)。甲基化改变了PP2A的构象,也改变了它的定位和B调节亚基的相互作用(6-8)。

  1. Janssens, V. and Goris, J. (2001) Biochem J 353, 417-39.
  2. Zolnierowicz, S. (2000) Biochem Pharmacol 60, 1225-35.
  3. Millward, T.A. et al. (1999) Trends Biochem Sci 24, 186-91.
  4. Chen, J. et al. (1992) Science 257, 1261-4.
  5. Turowski, P. et al. (1995) J Cell Biol 129, 397-410.
  6. Lee, J. et al. (1996) Proc Natl Acad Sci U S A 93, 6043-7.
  7. Tolstykh, T. et al. (2000) EMBO J 19, 5682-91.
  8. Yu, X.X. et al. (2001) Mol Biol Cell 12, 185-99.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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