Cell Signaling Technology

Product Pathways - Metabolism

MRP6/ABCC6 (D9D1F) Rabbit mAb #10666

No. Size Price
10666S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
10666 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 160-200 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MRP6/ABCC6 (D9D1F) Rabbit mAb recognizes endogenous levels of total MRP6 protein. This antibody also cross-reacts with unidentified proteins of 65 kDa and 135 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MRP6 protein.

Western Blotting

Western Blotting

Western blot analysis of membrane extracts from various tissues using MRP6/ABCC6 (D9D1F) Rabbit mAb.

Background

The multidrug resistance-associated protein 6 (MRP6, ABCC6) is a member of ATP-binding cassette (ABC) family transporters that move drugs and hydrophobic compounds across cell membranes. The MRP6 protein is expressed mainly in liver and kidney, and in other tissues to a lesser extent (1). Identified MRP6 substrates include the glutathione conjugate of N-ethylmaleimide (NEM-GS) and leukotriene C4 (LTC4), with more tentative MRP6 substrates under investigation (2,3). Research studies show that increased MRP6 expression correlates with induced cholesterol biosynthesis, which suggests that MRP6 may be involved in lipid and cholesterol homeostasis (4). A small isoform of MRP6 is up-regulated in HBV infected hepatocytes and protects the cells from apoptosis mediated by caspase 3 and caspase 8 (5,6). Mutations in the corresponding ABCC6 gene cause pseudoxanthoma elasticum (PXE), an autosomal recessive disorder that is characterized by the accumulation of mineralized and fragmented elastic fibers in the skin, eyes, and arteries (7,8). Mutations in ABCC6 also result in generalized arterial calcification of infancy, an ectopic calcification disease that lies along a spectrum of similar disorders with PXE (9).

  1. Kool, M. et al. (1999) Cancer Res 59, 175-82.
  2. Iliás, A. et al. (2002) J Biol Chem 277, 16860-7.
  3. Belinsky, M.G. et al. (2002) Cancer Res 62, 6172-7.
  4. Kuzaj, P. et al. (2014) Lipids Health Dis 13, 118.
  5. Ostuni, A. et al. (2013) FEBS Lett 587, 3058-62.
  6. Ostuni, A. et al. (2014) Protein Pept Lett 21, 413-8.
  7. Bergen, A.A. et al. (2000) Nat Genet 25, 228-31.
  8. Ringpfeil, F. et al. (2000) Proc Natl Acad Sci U S A 97, 6001-6.
  9. Nitschke, Y. et al. (2012) Am J Hum Genet 90, 25-39.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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