Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

β-Tubulin Blocking Peptide #1032


Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:


This peptide is used to block β-Tubulin (9F3) Rabbit mAb #2128 reactivity.

该多肽通常用于封闭β-Tubulin (9F3) Rabbit mAb #2128反应活性。

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks β-Tubulin (9F3) Rabbit mAb #2128 by immunohistochemistry.

使用反相HPLC和质谱仪分析评价该多肽的质量。该多肽在免疫组化(immunohistochemistry)实验中封闭β-Tubulin (9F3) Rabbit mAb #2128信号。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb #2128 in the presence of control peptide (left) or β-Tubulin Blocking Peptide (right). 使用β-Tubulin (9F3) Rabbit mAb #2128,免疫组化(immunohistochemistry)分析人类乳腺癌组织的石蜡切片,其分别孵育control peptide (左图)和β-Tubulin Blocking Peptide (右图)。


Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.


Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.

对于免疫组化(immunohistochemistry)实验,在总体积为100 μl反应体系中多肽的体积应是抗体体积的两倍。并且在将总反应体积加到切片至少30 minutes开始孵育多肽。抗体的推荐稀释度标注在产品说明书上。


The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).


  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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