Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

β-Tubulin Blocking Peptide #1032

βtubulin  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

This peptide is used to block β-Tubulin (9F3) Rabbit mAb #2128 reactivity.

该多肽通常用于封闭β-Tubulin (9F3) Rabbit mAb #2128反应活性。

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks β-Tubulin (9F3) Rabbit mAb #2128 by immunohistochemistry.

使用反相HPLC和质谱仪分析评价该多肽的质量。该多肽在免疫组化(immunohistochemistry)实验中封闭β-Tubulin (9F3) Rabbit mAb #2128信号。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb #2128 in the presence of control peptide (left) or β-Tubulin Blocking Peptide (right). 使用β-Tubulin (9F3) Rabbit mAb #2128,免疫组化(immunohistochemistry)分析人类乳腺癌组织的石蜡切片,其分别孵育control peptide (左图)和β-Tubulin Blocking Peptide (右图)。

Applications

Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.

在免疫组化(immunohistochemistry)实验中,使用一个封闭试剂可以评估这个抗体的特异性反应。

Directions for Use

For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.

对于免疫组化(immunohistochemistry)实验,在总体积为100 μl反应体系中多肽的体积应是抗体体积的两倍。并且在将总反应体积加到切片至少30 minutes开始孵育多肽。抗体的推荐稀释度标注在产品说明书上。

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架由三种类型的细胞质基质纤维组成:微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块,由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的,包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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