Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb #10001

Akt Substrate   Motif Antibody   PTMScan  

No. Size Price
10001S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
10001 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb recognizes endogenous proteins containing phospho-Ser/Thr preceded by Arg at positions -5 and -3 in a manner largely independent of the surrounding amino acid sequence. Minor cross-reactivity is observed for proteins that contain phospho-Ser/Thr preceded by Arg at position -3 only. No cross-reactivity is observed with the corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs.

Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb兔单抗识别包含-5、-3位是精氨酸的磷酸化丝氨酸/苏氨酸的内源性蛋白质,与周围序列无关。此抗体仅与-3位是精氨酸的磷酸化丝氨酸/苏氨酸的蛋白质有较弱的交叉反应。没有发现其能与相应的非磷酸化的序列或其它基序中的磷酸化丝氨酸/苏氨酸序列发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with an Akt substrate peptide library.

该单克隆抗体用Akt底物库肽段免疫动物制备。

Western Blotting

Western Blotting

Western blot analysis of extracts from MKN-45 cells, untreated or treated with c-Met kinase inhibitor SU11274 (1 µM, 2 hr), using Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb. Western blot image was obtained using the Odyssey® Infrared Imaging System (LI-COR® Biotechnology).

对未处理(-)或c-Met激酶抑制剂SU11274(1 uM,2小时)处理的MKN-45细胞抽提液使用Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb兔单抗进行Western blot分析。Western Blot图片采集使用Odyssey® Infrared Imaging System (LI-COR® Biotechnology)。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated or treated with Human Epidermal Growth Factor (hEGF) #8916 (10 ng/ml, 20 min), using Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb (upper), or Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (lower). Western blot images were obtained using the Odyssey® Infrared Imaging System (LI-COR® Biotechnology).

对未处理(-)或人表皮生长因子(hEGF) #8916 (10 ng/ml, 20 min)处理的A-431细胞抽提液使用Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb(上)和Phospho-Akt (Ser473) (D9E) XP庐 Rabbit mAb #4060(下)兔单抗进行Western blot分析。Western Blot图片采集使用Odyssey® Infrared Imaging System (LI-COR® Biotechnology)。

Background

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

精氨酸导向的激酶或AGC家族激酶是很重要的激酶,包括cAMP依赖的蛋白激酶(PKA)、cGMP依赖的蛋白激酶(PKG)、蛋白激酶C、Akt和RSK。这些激酶有相同的底物特异性,-3位上有精氨酸的丝氨酸和苏氨酸(1,2)。Akt在介导细胞内关键反应中扮演重要角色,包括细胞生长和生存、血管生成和转录调节(3-5)。目前已经发现一些Akt底物(如GSK-3、Bad、caspase-9),但还有很多重要底物等待发现。Akt磷酸化保守序列-5、-3位是精氨酸的丝氨酸/苏氨酸(6)。磷酸化Akt底物特异性抗体由Cell Signaling Technology公司研发,用于研究Akt磷酸化调节和精氨酸导向的激酶,也可以用于高通量药物发现。

  1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  3. Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.
  4. Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.
  5. Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.
  6. Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

LI-COR is a registered trademark of LI-COR, Inc.

Odyssey is a registered trademark of LI-COR, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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