Cell Signaling Technology

PathScan® 化学发光夹心ELISA试剂盒

新的PathScan® 化学发光夹心ELISA试剂盒比传统的显色检测系统具有更宽的动态范围和更强的灵敏性.这些试剂盒提供小体积的微孔板,可以在使用小体积样本时保持强信号和灵敏性。

Phospho-Akt1 (Ser473)

Levels of Phospho-Akt1 (Ser473) in PDGF-treated and untreated NIH/3T3 cells

图中展示的是PDGF处理的和未处理的NIH/3T3细胞中磷酸化Akt1(Ser473)的水平。由化学发光读数决定的信号强度以RLUs(相对光单位)给出。这个信号与加入的蛋白裂解物的含量成正比。

插入的图表明即使在蛋白浓度很低的情况下,PDGF处理的结果也具有高灵敏性并呈线性。

图1: 未处理的和PDGF处理的NIH/3T3细胞裂解物中蛋白浓度的关系以及化学发光底物产生的瞬时光强度。细胞(80%密度)用PDGF(50ng/ml)处理并在37ºC孵育20分钟后裂解。插入的表对应的是阴影区域。

化学发光试剂盒

No. Name
No. Name
12116 PathScan® Phospho-Acetyl-CoA Carboxylase (Ser79) Chemiluminescent Sandwich ELISA Kit
12321 PathScan® Phospho-Ret (panTyr) Chemiluminescent Sandwich ELISA Kit
7020 PathScan® Phospho-ALK (Tyr1604) Chemiluminescent Sandwich ELISA Kit
7021 PathScan® Phospho-FLT3 (Tyr591) Chemiluminescent Sandwich ELISA Kit
7024 PathScan® Phospho-TrkA (Tyr490) Chemiluminescent Sandwich ELISA Kit
7027 PathScan® Phospho-c-Jun (Ser63) Chemiluminescent Sandwich ELISA Kit
7029 PathScan® Phospho-MEK1 (Ser217/221) Chemiluminescent Sandwich ELISA Kit
7036 PathScan® Phospho-EGF Receptor (Tyr1068) Chemiluminescent Sandwich ELISA Kit
7084 PathScan® Total ALK Chemiluminescent Sandwich ELISA Kit
7087 PathScan® Phospho-TrkB (panTyr) Chemiluminescent Sandwich ELISA Kit
7132 PathScan® Total Akt1 Chemiluminescent Sandwich ELISA Kit
7134 PathScan® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit
7135 PathScan® Phospho-Akt (Thr308) Chemiluminescent Sandwich ELISA Kit
7149 PathScan® Phospho-Stat3 (Tyr705) Chemiluminescent Sandwich ELISA Kit
7153 PathScan® Phospho-p70 S6 Kinase (Thr389) Chemiluminescent Sandwich ELISA Kit
7297 PathScan® Total EGF Receptor Chemiluminescent Sandwich ELISA Kit
7316 PathScan® Phospho-S6 Ribosomal Protein (Ser235/236) Chemiluminescent Sandwich ELISA Kit
7337 PathScan® Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit
7792 PathScan® Phospho-AMPKα (Thr172) Chemiluminescent Sandwich ELISA Kit
7849 PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Chemiluminescent Sandwich ELISA Kit
8026 PathScan® Phospho-Stat3 (Ser727) Chemiluminescent Sandwich ELISA Kit

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